Frontiers in Bioengineering and Biotechnology

Original Research

Published on 16 Sep 2025

Viable cell sorting by fluidized bed centrifugation enables novel cultivation strategies

in Bioprocess Engineering

Martin Saballus
Lucas Nik Reger
Robin Obser
Julia Niemann
Rene H. Wijffels
Dirk E. Martens
Markus Kampmann

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1667343

2,557 views

Original Research

Published on 15 Sep 2025

Metabolome profile and microbial community structure of Cenchrus fungigraminus silage under different moisture gradients

in Bioprocess Engineering

Xiaohan Hou
Xiaohui Chu
Yang Yang
Xue Xiao
Qiongmei Niu
Guilian Shan

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1657657

1,537 views

Original Research

Published on 12 Sep 2025

Removal of hexavalent chromium by a microbial mat from a mining site under anaerobic conditions

in Bioprocess Engineering

Mohammad Tariq Ali Khan
Sumaiya Said Al-Siyabi
Hamada E. Ali
Raeid M. M. Abed

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1585237

1,927 views

Brief Research Report

Published on 09 Sep 2025

Selective and mild oxidation of α-hydroxyl groups in lignin by 2-iodoxybenzoic acid

in Bioprocess Engineering

Fei Jiang
Zhiming Dong
Xiaoli Lou
Zhuonan Zhang
Li Shuai
Chengke Zhao

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1654739

1,686 views

Perspective

Published on 03 Sep 2025

Isolator-based point-of-care manufacturing: a practical solution for GMP-compliant cell and extracellular vesicles therapy production

in Bioprocess Engineering

Yu-Sung Chiu
Kun-Liang Wang
Kun-Lieh Wu

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1644318

2,885 views
1 citation

Mini Review

Published on 21 Aug 2025

Identification of critical process parameters and quality attributes for bioreactor-based expansion of human MSCs

in Bioprocess Engineering

Laura Herbst
Bastian Nießing
Robert H. Schmitt

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1608194

2,796 views

Original Research

Published on 21 Aug 2025

Raman-based PAT for multi-attribute monitoring during VLP recovery by dual-stage CFF: attribute-specific spectral preprocessing for model transfer

in Bioprocess Engineering

Annabelle Dietrich
Luca Heim
Jürgen Hubbuch

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1631807

1,968 views

Original Research

Published on 06 Aug 2025

Development and optimization of an ammonia removal strategy for sustainable recycling of cell culture spent media in cultivated meat production: from concept to implementation

in Bioprocess Engineering

Babak Pakbin
Armaghan Amanipour
Arian Amirvaresi
Arash Shahsavari
Reza Ovissipour

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1617115

2,934 views
1 citation

Original Research

Published on 01 Aug 2025

Evaluation of a single-use bioartificial liver (BAL) biocartridge consisting of cryopreservable alginate encapsulated liver cell spheroids as a component of HepatiCan™, a novel bioartificial liver device

in Bioprocess Engineering

Eloy Erro
Tom Brookshaw
Barry Fuller
Sweta Chandel
Joana Mendonca da Silva
Elizaveta Zotova
Sherri-Ann Chalmers
Alfie Watt
Clare Selden

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1572254

3,388 views

Original Research

Published on 01 Aug 2025

Enhanced cancer cell sorting using lab-on-a-disk pattern design with magnetic and centrifugal forces

in Bioprocess Engineering

Bill Cheng
Wei-Cheng Chao
Yi-Han Chen
Yao-Tsung Lin

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1611313

1,733 views

Review

Published on 31 Jul 2025

Advances in alginate biosynthesis: regulation and production in Azotobacter vinelandii

in Bioprocess Engineering

Belén Ponce
Agustín Zamora-Quiroz
Ernesto González
Rodrigo Andler
Alvaro Díaz-Barrera

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1593893

3,566 views
1 citation

Original Research

Published on 31 Jul 2025

Automated, aseptic sampling with small-volume capacity from microbioreactors for cell therapy process analysis

in Bioprocess Engineering

Zhi Xian Chan
Shruthi Pandi Chelvam
Wei-Xiang Sin
Denise Bei Lin Teo
Ahmad Amirul Bin Abdul Rahim
Ying Ying Wu
Dan Liu
Michael E. Birnbaum
Derrick Yong
Rajeev J. Ram

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1612648

3,024 views

Original Research

Published on 30 Jul 2025

Anomaly detection and removal strategies for in-line permittivity sensor signal used in bioprocesses

in Bioprocess Engineering

Emils Bolmanis
Selina Uhlendorff
Miriam Pein-Hackelbusch
Vytautas Galvanauskas
Oskars Grigs

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1609369

2,034 views

Original Research

Published on 23 Jul 2025

Adaptive responses of Gordonia alkanivorans IEGM 1277 to the action of meloxicam and its efficient biodegradation

in Bioprocess Engineering

Semyon Tyan
Nadezhda Kostrikina
Vladimir Sorokin
Andrey Mulyukin
Irina Ivshina

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1603975

1,939 views
1 citation

Original Research

Published on 18 Jul 2025

Cell specific perfusion rates drive growth dynamics and metabolism in CHO N-1 perfusion processes independent of perfusion rate control method

in Bioprocess Engineering

Julia Walther
Tiago Ribeiro da Costa
Lydia Winkler
Jochen Schaub
Tobias Habicher

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1608889

3,249 views

Original Research

Published on 16 Jul 2025

Hyaluronidases improve the hyaluronic acid yield during the fermentation of Streptococcus zooepidemicus

in Bioprocess Engineering

Chuan-Li Kang
De-Qiang Zheng
Zhi-Yuan Yao
Kang Yang
Yuxue Zhao
Zihan Mao
Yang Liu
Haijun Li
Jin-Song Gong
Lei Liu

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1625009

2,552 views

Review

Published on 10 Jul 2025

Insights into bone and cartilage responses to pulsed electromagnetic field stimulation: a review with quantitative comparisons

in Bioprocess Engineering

Beatrice Masante
Stefano Gabetti
Joao C. Silva
Giovanni Putame
Simone Israel
Cristina Bignardi
Diana Massai

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1557572

4,560 views
3 citations

Review

Published on 09 Jul 2025

Recent advances and future directions on GLA-producing organisms

in Bioprocess Engineering

Mojgan Latifi
Foroozan Jalali Bidgoli
Helia Hajihassani
Danial Hassani
Pär K. Ingvarsson
Naser Farrokhi

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1567840

3,730 views
2 citations

Original Research

Published on 07 Jul 2025

Design, synthesis, and anti-inflammatory potential of PROTAC drug molecules based on fondaparinux sodium

in Bioprocess Engineering

Ruoxuan Wu
Tianji Zhang
Siran Zhao
Marco Maccarana
Jin-Ping Li
Chao Li
Hui Cao

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1597344

2,795 views

Methods

Published on 27 Jun 2025

Optimizing protein yield from growing deer antlers

in Bioprocess Engineering

Nicolás Alegría-Aravena
María-Pilar López-Garrido
Josefa Quiroz-Troncoso
Raquel González-Martos
Marta Sánchez-Díez
Clara Gavira-O’Neill
Andrés J. García-Díaz
Tomás Landete-Castillejos
Carmen Ramírez-Castillejo
Louis Chonco

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1612239

2,242 views

Brief Research Report

Published on 05 Jun 2025

Unlocking efficiency in column chromatography with packed bed supporting inserts

in Bioprocess Engineering

Romone M. Fancy
David H. Abraham
Matthew R. Taylor
Cole McMullin
Nicholas S. Brann
Daniel M. Bailey
David I. Brown
Heather Bethea Horne
Leslie S. Wolfe

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1613174

3,331 views

Review

Published on 04 Jun 2025

Enzymatic approaches to nicotinic acid synthesis: recent advances and future prospects

in Bioprocess Engineering

Rahul Vikram Singh
Vipin Chandra Kalia
Krishika Sambyal
Bakul Singh
Anurag Kumar
Jung-Kul Lee

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1585736

7,355 views
2 citations

Original Research

Published on 02 Jun 2025

Optimising and adapting perfusion feeds in serum-free medium to intensify CAR-T cell expansion in stirred-tank bioreactors

in Bioprocess Engineering

Pierre Springuel
Tiffany Hood
Fern Slingsby
Timo Schmidberger
Nicola Bevan
Noushin Dianat
Julia Hengst
Qasim A. Rafiq

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1593895

4,565 views
2 citations

Original Research

Published on 30 May 2025

Perfusion process with tangential flow filtration for oncolytic VSV-GP production

in Bioprocess Engineering

Orsolya Hamusics
Anja Wittmann
Katrin Hasler
Sabrina Müller
Daniel Birk
Ingo H. Gorr
Alexander Brix
Jorge Soza-Ried

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1588293

2,269 views
1 citation

1,003 articles

articles

Viable cell sorting by fluidized bed centrifugation enables novel cultivation strategies

Metabolome profile and microbial community structure of Cenchrus fungigraminus silage under different moisture gradients

Removal of hexavalent chromium by a microbial mat from a mining site under anaerobic conditions

Selective and mild oxidation of α-hydroxyl groups in lignin by 2-iodoxybenzoic acid

Isolator-based point-of-care manufacturing: a practical solution for GMP-compliant cell and extracellular vesicles therapy production

Identification of critical process parameters and quality attributes for bioreactor-based expansion of human MSCs

Raman-based PAT for multi-attribute monitoring during VLP recovery by dual-stage CFF: attribute-specific spectral preprocessing for model transfer

Development and optimization of an ammonia removal strategy for sustainable recycling of cell culture spent media in cultivated meat production: from concept to implementation

Evaluation of a single-use bioartificial liver (BAL) biocartridge consisting of cryopreservable alginate encapsulated liver cell spheroids as a component of HepatiCan™, a novel bioartificial liver device

Enhanced cancer cell sorting using lab-on-a-disk pattern design with magnetic and centrifugal forces

Advances in alginate biosynthesis: regulation and production in Azotobacter vinelandii

Automated, aseptic sampling with small-volume capacity from microbioreactors for cell therapy process analysis

Anomaly detection and removal strategies for in-line permittivity sensor signal used in bioprocesses

Adaptive responses of Gordonia alkanivorans IEGM 1277 to the action of meloxicam and its efficient biodegradation

Cell specific perfusion rates drive growth dynamics and metabolism in CHO N-1 perfusion processes independent of perfusion rate control method

Hyaluronidases improve the hyaluronic acid yield during the fermentation of Streptococcus zooepidemicus

Insights into bone and cartilage responses to pulsed electromagnetic field stimulation: a review with quantitative comparisons

Recent advances and future directions on GLA-producing organisms

Design, synthesis, and anti-inflammatory potential of PROTAC drug molecules based on fondaparinux sodium

Optimizing protein yield from growing deer antlers

Unlocking efficiency in column chromatography with packed bed supporting inserts

Enzymatic approaches to nicotinic acid synthesis: recent advances and future prospects

Optimising and adapting perfusion feeds in serum-free medium to intensify CAR-T cell expansion in stirred-tank bioreactors

Perfusion process with tangential flow filtration for oncolytic VSV-GP production