Frontiers in Bioengineering and Biotechnology

Original Research

Published on 25 Jul 2025

Regulation of RHBDD1 in the invasion of esophageal cancer cells via ELK3/Wnt/β-catenin signaling pathway

in Cell and Gene Therapy

Bing Xu
Hui Chen
Xinchen Sun
Hongyan Cheng
Kavimbi Chipusu
Wei Hua
Tingting Chen

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1604859

2,293 views
1 citation

Original Research

Published on 23 Jul 2025

Comprehensive analysis of pyroptosis-related genes in psoriasis and targeted gene editing of CASP1 and CASP5 using lipid nanoparticles to alleviate skin inflammation

in Cell and Gene Therapy

Gexiao Xu
Guanyi Ma
Jiachen Sun
Xiaoyan Yu
Jie Sun
Bing Gao

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1639869

3,316 views

Original Research

Published on 14 Jul 2025

A pilot study of healing critical-sized calvarial defects by LL-37-generated monoosteophils

in Cell and Gene Therapy

Keith Le
Huinan Liu
Chaoxing Zhang
Zhuo Li
Tove Olafsen
Yuman Fong
John E. Shively
Zhifang Zhang

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1583496

1,970 views

Original Research

Published on 04 Jul 2025

Cell retention in scalable, perfusion-based mesenchymal stem cell expansion processes: a proof of concept

in Cell and Gene Therapy

Samuel Lukas Schneider
Abiram Gopalakrishnan
Misha Alexander Teale
Regine Eibl

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1611703

2,703 views

Correction

Published on 26 Jun 2025

Correction: An engineered adipose formulation decreases hepatic inflammation and fibrosis in a rodent model of metabolic dysfunction-associated steatotic liver disease

in Cell and Gene Therapy

Youngshim Choi
Yinyan Ma
Samson Tom
Alla Danilkovitch
Liqing Yu

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1642526

1,091 views

Original Research

Published on 25 Jun 2025

Closed-system manufacturing of therapeutic NK cells using automated cell enrichment and concentration processes enables scalable, robust and cost-effective solutions

in Cell and Gene Therapy

Nina Kok
Zoë Dekkers
Anica Loos
Thomas Bos
Puck van den Broek
Inge Post
Bertine Niessen
Jan Spanholtz
Volker Huppert
Monica Raimo

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1586912

3,939 views
1 citation

Review

Published on 23 Jun 2025

Lung cancer vaccine strategies: exploring the spectrum from traditional to RNA-based platforms

in Cell and Gene Therapy

Alireza Pazoki
Sepehr Dadfar
Atefe Alirezaee
Valentyn Oksenych
Dariush Haghmorad

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1617352

4,043 views
1 citation

Review

Published on 13 Jun 2025

Mesenchymal stem cells derived extracellular vesicles for chronic kidney disease: pleiotropic mechanisms of actions of a versatile therapy

in Cell and Gene Therapy

Elena Ceccotti
Marco Quaglia
Giovanni Camussi
Stefania Bruno

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1612193

5,363 views
2 citations

Review

Published on 09 Jun 2025

Chondroitinase ABC in spinal cord injury: advances in delivery strategies and therapeutic synergies

in Cell and Gene Therapy

Rachel Santana Cunha
Erik Aranha Rossi
Thaís Alves de Santana
Zaquer Suzana Munhoz Costa-Ferro
Bruno Solano de Freitas Souza

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1604502

4,731 views
1 citation

Original Research

Published on 06 Jun 2025

An engineered adipose formulation decreases hepatic inflammation and fibrosis in a rodent model of metabolic dysfunction-associated steatotic liver disease

in Cell and Gene Therapy

Youngshim Choi
Yinyan Ma
Samson Tom
Alla Danilkovitch
Liqing Yu

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1579062

2,801 views

Review

Published on 30 May 2025

Challenges and future perspectives in using mesenchymal stem cells for efficient bone fracture healing

in Cell and Gene Therapy

Dong Han
Weiwei Liu
Jinpeng Gong
Yupeng Ma
Zhengwen Sun

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1568914

4,291 views
1 citation

Perspective

Published on 29 May 2025

Challenges in lentiviral vector production: retro-transduction of producer cell lines

in Cell and Gene Therapy

Maximilian Klimpel
Christin Obert
Monica Terrao
Parameswari Singh
Haiyan Wei
Chao-Guang Chen
Andreas Gille
Silke Wissing
Holger Laux

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1569298

5,293 views
2 citations

Original Research

Published on 27 May 2025

Sustained capillary enlargement induced by angiogenic gene therapy does not support post-ischemic muscle recovery of hyperlipidemic mice

in Cell and Gene Therapy

Galina Wirth
Greta Juusola
Hanne Laakso
Nihay Laham-Karam
Seppo Ylä-Herttuala
Petra Korpisalo

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1512962

1,819 views

Review

Published on 20 May 2025

Bottom-up Biomaterial strategies for creating tailored stem cells in regenerative medicine

in Cell and Gene Therapy

Brenda Cruz-Gonzalez
Ellie Johandes
Dominique Gramm
Donny Hanjaya-Putra

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1581292

4,332 views
4 citations

Review

Published on 14 May 2025

Extracellular vesicles as vital players in drug delivery: a focus on clinical disease treatment

in Cell and Gene Therapy

Tiangang Chen
Dan Chen
Wanting Su
Jianlan Liang
Xiangning Liu
Mingxiang Cai

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1600227

5,806 views
7 citations

Review

Published on 08 May 2025

Harnessing CRISPR potential for intervertebral disc regeneration strategies

in Cell and Gene Therapy

Catarina Milheiro
Maria L. Moura
Mario Amendola
Mário A. Barbosa
Joana Caldeira

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1562412

4,301 views
1 citation

Correction

Published on 06 May 2025

Corrigendum: Screening for MicroRNA combination with engineered exosomes as a new tool against osteosarcoma in elderly patients

in Cell and Gene Therapy

Jiyu Han
Zitong Zhao
Yanhong Wang
Tao Yu
Daqian Wan

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1615190

1,530 views

Original Research

Published on 06 May 2025

Involvement of long non-coding RNA (lncRNA) MALAT1 in shear stress regulated adipocyte differentiation

in Cell and Gene Therapy

Justin Caron
Marjan Ghanbariabdolmaleki
Madison Marino
Chong Qiu
Bo Wang
Michael Mak
Shue Wang

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1570518

2,698 views

Review

Published on 24 Apr 2025

Mesenchymal stem cells and their extracellular vesicles: new therapies for cartilage repair

in Cell and Gene Therapy

Hongwei Yang
Haochen Yang
Qin Wang
Hanzhen Ji
Tianmei Qian
Yusen Qiao
Junfeng Shi
Meng Cong

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1591400

4,314 views
6 citations

Original Research

Published on 04 Apr 2025

Autofluorescence lifetime imaging classifies human B and NK cell activation state

in Cell and Gene Therapy

Rebecca L. Schmitz
Jeremiah M. Riendeau
Kelsey E. Tweed
Peter Rehani
Kayvan Samimi
Dan L. Pham
Isabel Jones
Elizabeth M. Maly
Emmanuel Contreras Guzman
Matthew H. Forsberg

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1557021

3,077 views
1 citation

Review

Published on 03 Apr 2025

Extracellular vesicles: innovative cell-free solutions for wound repair

in Cell and Gene Therapy

Hanne Eerdekens
Elke Pirlet
Sarah Willems
Annelies Bronckaers
Paula M. Pincela Lins

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1571461

4,240 views
5 citations

Original Research

Published on 21 Mar 2025

Lymphoblastoid and Jurkat cell lines are useful surrogate in developing a CRISPR-Cas9 method to correct leukocyte adhesion deficiency genomic defect

in Cell and Gene Therapy

Ahmad R. Ramadan
Noureddine Ben Khalaf
Khaled Trabelsi
Halla Bakheit
Imen Ben-Mustapha
Mohamed-Ridha Barbouche
M-Dahmani Fathallah

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1548227

2,511 views
1 citation

Original Research

Published on 20 Mar 2025

Towards propagation of epidermal cells for wound repair: glass, as cell culture substrate, enhances proliferation and migration of human keratinocytes

in Cell and Gene Therapy

Hady Shahin
Ingrid Steinvall
Folke Sjöberg
Moustafa Elmasry
Ahmed El-Serafi

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1547044

2,108 views
1 citation

Opinion

Published on 19 Mar 2025

CGT 4.0: a distant dream or inevitable future? Smart process automation is critical to make efficient scalability of CGT manufacturing a reality

in Cell and Gene Therapy

Aleksander Szarzynski
Oliver Spadiut
Matthias Reisbeck
Gerhard Jobst
Rachel L. Paterson
Anna Kamenskaya
Emilie Gateau
Hanna P. Lesch
Luc Henry
Bence Kozma

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1563878

7,531 views
2 citations