Frontiers in Bioengineering and Biotechnology

Original Research

Published on 17 Dec 2021

Using Unnatural Protein Fusions to Engineer a Coenzyme Self-Sufficiency System for D-Phenyllactic Acid Biosynthesis in Escherichia coli

in Synthetic Biology

Zhao Qin
Dan Wang
Ruoshi Luo
Tinglan Li
Xiaochao Xiong
Peng Chen

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2021.795885

4,540 views
8 citations

Original Research

Published on 17 Dec 2021

Functional Trimeric SARS-CoV-2 Envelope Protein Expressed in Stable CHO Cells

in Synthetic Biology

Patrick Mayrhofer
Monika Hunjadi
Renate Kunert

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2021.779359

6,196 views
5 citations

Review

Published on 17 Dec 2021

New Insights Into the Biosynthesis of Typical Bioactive Components in the Traditional Chinese Medicinal Fungus Cordyceps militaris

in Synthetic Biology

Xiuyun Wu
Tao Wu
Ailin Huang
Yuanyuan Shen
Xuanyu Zhang
Wenjun Song
Suying Wang
Haihua Ruan

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2021.801721

10,165 views
24 citations

Perspective

Published on 15 Dec 2021

Overstatements and Understatements in the Debate on Synthetic Biology, Bioterrorism and Ethics

in Synthetic Biology

Anders Melin

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2021.703735

6,159 views
5 citations

Original Research

Published on 15 Dec 2021

Levulinic Acid-Inducible and Tunable Gene Expression System for Methylorubrum extorquens

in Synthetic Biology

Chandran Sathesh-Prabu
Young Shin Ryu
Sung Kuk Lee

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2021.797020

3,953 views
7 citations

Editorial

Published on 15 Dec 2021

Editorial: Multi-Omics Technologies for Optimizing Synthetic Biomanufacturing

in Synthetic Biology

Young-Mo Kim
Christopher J. Petzold
Eduard J. Kerkhoven
Scott E. Baker

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2021.818010

4,092 views
2 citations

Original Research

Published on 15 Dec 2021

Construction of an IS-Free Corynebacterium glutamicum ATCC 13 032 Chassis Strain and Random Mutagenesis Using the Endogenous ISCg1 Transposase

in Synthetic Biology

Marten Linder
Markus Haak
Angela Botes
Jörn Kalinowski
Christian Rückert

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2021.751334

4,819 views
10 citations

Review

Published on 14 Dec 2021

Strategies to Enhance Periplasmic Recombinant Protein Production Yields in Escherichia coli

in Synthetic Biology

Alexandros Karyolaimos
Jan-Willem de Gier

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2021.797334

28,605 views
36 citations

Review

Published on 13 Dec 2021

HEK293 Cell Line as a Platform to Produce Recombinant Proteins and Viral Vectors

in Synthetic Biology

Evan Tan
Cara Sze Hui Chin
Zhi Feng Sherman Lim
Say Kong Ng

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2021.796991

127,855 views
191 citations

Brief Research Report

Published on 10 Dec 2021

Introduction of Modified BglBrick System in Lactococcus lactis for Straightforward Assembly of Multiple Gene Cassettes

in Synthetic Biology

Tina Vida Plavec
Tim Ključevšek
Aleš Berlec

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2021.797521

2,973 views
7 citations

Brief Research Report

Published on 09 Dec 2021

Improved Functional Expression of Cytochrome P450s in Saccharomyces cerevisiae Through Screening a cDNA Library From Arabidopsis thaliana

in Synthetic Biology

Lihong Jiang
Chang Dong
Tengfei Liu
Yi Shi
Handing Wang
Zeng Tao
Yan Liang
Jiazhang Lian

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2021.764851

6,553 views
11 citations

Original Research

Published on 08 Dec 2021

Bamboo Shark as a Small Animal Model for Single Domain Antibody Production

in Synthetic Biology

Likun Wei
Meiniang Wang
Haitao Xiang
Yuan Jiang
Jinhua Gong
Dan Su
M. A. R. Al Azad
Hongming Dong
Limin Feng
Jiajun Wu

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2021.792111

11,138 views
25 citations

Original Research

Published on 03 Dec 2021

Highly Sensitive Whole-Cell Biosensor for Cadmium Detection Based on a Negative Feedback Circuit

in Synthetic Biology

Guangbao Zhang
Shuting Hu
Xiaoqiang Jia

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2021.799781

6,584 views
19 citations

Review

Published on 03 Dec 2021

Biofilm Biology and Engineering of Geobacter and Shewanella spp. for Energy Applications

in Synthetic Biology

Yidan Hu
Yinghui Wang
Xi Han
Yawei Shan
Feng Li
Liang Shi

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2021.786416

18,476 views
80 citations

Review

Published on 30 Nov 2021

Circular RNA: Biosynthesis in vitro

in Synthetic Biology

Xinjie Chen
Yuan Lu

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2021.787881

35,553 views
65 citations

Brief Research Report

Published on 30 Nov 2021

Evaluation of the Anti-Inflammatory and Anti-Oxidative Effects of Therapeutic Human Lactoferrin Fragments

in Synthetic Biology

Yu Pan
Zhao Liu
Yijie Wang
Linshen Zhang
Niying Chua
Lei Dai
Jun Chen
Chun Loong Ho

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2021.779018

5,758 views
14 citations

Editorial

Published on 29 Nov 2021

Editorial: Cell-Free Synthetic Biology

in Synthetic Biology

Jian Li
Yong-Chan Kwon
Yuan Lu
Simon J. Moore

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2021.799122

5,387 views
3 citations

Original Research

Published on 25 Nov 2021

Characterization and Heterologous Expression of UDP-Glucose 4-Epimerase From a Hericium erinaceus Mutant with High Polysaccharide Production

in Synthetic Biology

Gen Zou
Juanbao Ren
Di Wu
Henan Zhang
Ming Gong
Wen Li
Jingsong Zhang
Yan Yang

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2021.796278

4,440 views
9 citations

Original Research

Published on 24 Nov 2021

Gain of Spontaneous clpX Mutations Boosting Motility via Adaption to Environments in Escherichia coli

in Synthetic Biology

Bingyu Li
Chaofan Hou
Xian Ju
Yong Feng
Zhi-Qiang Ye
Yunzhu Xiao
Mingyao Gu
Chunxiang Fu
Chaoliang Wei
Conghui You

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2021.772397

6,686 views
4 citations

Brief Research Report

Published on 23 Nov 2021

De Novo Production of Glycyrrhetic Acid 3-O-mono-β-D-glucuronide in Saccharomyces cerevisiae

in Synthetic Biology

Ying Huang
Dan Jiang
Guangxi Ren
Yan Yin
Yifan Sun
Tengfei Liu
Chunsheng Liu

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2021.709120

4,432 views
13 citations

Original Research

Published on 22 Nov 2021

Heterotrophically Ultrahigh-Cell-Density Cultivation of a High Protein-Yielding Unicellular Alga Chlorella With a Novel Nitrogen-Supply Strategy

in Synthetic Biology

Quan Xu
Guoli Hou
Jianping Chen
Hongxia Wang
Li Yuan
Danxiang Han
Qiang Hu
Hu Jin

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2021.774854

7,936 views
31 citations

Original Research

Published on 19 Nov 2021

Goals as Emergent Autopoietic Processes

in Synthetic Biology

Tomas Veloz

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2021.720652

4,061 views
7 citations

Original Research

Published on 19 Nov 2021

Construction of an Acetate Metabolic Pathway to Enhance Electron Generation of Engineered Shewanella oneidensis

in Synthetic Biology

Junqi Zhang
Zheng Chen
Changjiang Liu
Jianxun Li
Xingjuan An
Deguang Wu
Xi Sun
Baocai Zhang
Longping Fu
Feng Li

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2021.757953

6,498 views
18 citations

Original Research

Published on 18 Nov 2021

Genomics Characterization of an Engineered Corynebacterium glutamicum in Bioreactor Cultivation Under Ionic Liquid Stress

in Synthetic Biology

Deepanwita Banerjee
Thomas Eng
Yusuke Sasaki
Aparajitha Srinivasan
Asun Oka
Robin A. Herbert
Jessica Trinh
Vasanth R. Singan
Ning Sun
Dan Putnam

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2021.766674

5,941 views
9 citations

785 articles

articles

Using Unnatural Protein Fusions to Engineer a Coenzyme Self-Sufficiency System for D-Phenyllactic Acid Biosynthesis in Escherichia coli

Functional Trimeric SARS-CoV-2 Envelope Protein Expressed in Stable CHO Cells

New Insights Into the Biosynthesis of Typical Bioactive Components in the Traditional Chinese Medicinal Fungus Cordyceps militaris

Overstatements and Understatements in the Debate on Synthetic Biology, Bioterrorism and Ethics

Levulinic Acid-Inducible and Tunable Gene Expression System for Methylorubrum extorquens

Editorial: Multi-Omics Technologies for Optimizing Synthetic Biomanufacturing

Construction of an IS-Free Corynebacterium glutamicum ATCC 13 032 Chassis Strain and Random Mutagenesis Using the Endogenous ISCg1 Transposase

Strategies to Enhance Periplasmic Recombinant Protein Production Yields in Escherichia coli

HEK293 Cell Line as a Platform to Produce Recombinant Proteins and Viral Vectors

Introduction of Modified BglBrick System in Lactococcus lactis for Straightforward Assembly of Multiple Gene Cassettes

Improved Functional Expression of Cytochrome P450s in Saccharomyces cerevisiae Through Screening a cDNA Library From Arabidopsis thaliana

Bamboo Shark as a Small Animal Model for Single Domain Antibody Production

Highly Sensitive Whole-Cell Biosensor for Cadmium Detection Based on a Negative Feedback Circuit

Biofilm Biology and Engineering of Geobacter and Shewanella spp. for Energy Applications

Circular RNA: Biosynthesis in vitro

Evaluation of the Anti-Inflammatory and Anti-Oxidative Effects of Therapeutic Human Lactoferrin Fragments

Editorial: Cell-Free Synthetic Biology

Characterization and Heterologous Expression of UDP-Glucose 4-Epimerase From a Hericium erinaceus Mutant with High Polysaccharide Production

Gain of Spontaneous clpX Mutations Boosting Motility via Adaption to Environments in Escherichia coli

De Novo Production of Glycyrrhetic Acid 3-O-mono-β-D-glucuronide in Saccharomyces cerevisiae

Heterotrophically Ultrahigh-Cell-Density Cultivation of a High Protein-Yielding Unicellular Alga Chlorella With a Novel Nitrogen-Supply Strategy

Goals as Emergent Autopoietic Processes

Construction of an Acetate Metabolic Pathway to Enhance Electron Generation of Engineered Shewanella oneidensis

Genomics Characterization of an Engineered Corynebacterium glutamicum in Bioreactor Cultivation Under Ionic Liquid Stress