Frontiers in Genetics | Genomic Assay Technology

Original Research

Published on 30 Apr 2019

Biological Characteristics of Severe Combined Immunodeficient Mice Produced by CRISPR/Cas9-Mediated Rag2 and IL2rg Mutation

in Genomic Assay Technology

Yong Zhao
Peijuan Liu
Zhiqian Xin
Changhong Shi
Yinlan Bai
Xiuxuan Sun
Ya Zhao
Xiaoya Wang
Li Liu
Xuan Zhao

Frontiers in Genetics

doi 10.3389/fgene.2019.00401

10,419 views
19 citations

Original Research

Published on 30 Apr 2019

Enhancement of Precise Gene Editing by the Association of Cas9 With Homologous Recombination Factors

in Genomic Assay Technology

Ngoc-Tung Tran
Sanum Bashir
Xun Li
Jana Rossius
Van Trung Chu
Klaus Rajewsky
Ralf Kühn

Frontiers in Genetics

doi 10.3389/fgene.2019.00365

22,301 views
64 citations

Original Research

Published on 26 Apr 2019

Increasing Cytosine Base Editing Scope and Efficiency With Engineered Cas9-PmCDA1 Fusions and the Modified sgRNA in Rice

in Genomic Assay Technology

Ying Wu
Wen Xu
Feipeng Wang
Si Zhao
Feng Feng
Jinling Song
Chengwei Zhang
Jinxiao Yang

Frontiers in Genetics

doi 10.3389/fgene.2019.00379

8,166 views
39 citations

Original Research

Published on 26 Apr 2019

GhWRKY6 Acts as a Negative Regulator in Both Transgenic Arabidopsis and Cotton During Drought and Salt Stress

in Genomic Assay Technology

Zhi Li
Lei Li
Kehai Zhou
Yihao Zhang
Xiao Han
Yanpeng Din
Xiaoyang Ge
Wenqiang Qin
Peng Wang
Fuguang Li

Frontiers in Genetics

doi 10.3389/fgene.2019.00392

6,672 views
53 citations

Original Research

Published on 18 Apr 2019

sgRNA-shRNA Structure Mediated SNP Site Editing on Porcine IGF2 Gene by CRISPR/StCas9

in Genomic Assay Technology

Yongsen Sun
Nana Yan
Lu Mu
Bing Sun
Jingrong Deng
Yuanyuan Fang
Simin Shao
Qiang Yan
Furong Han
Zhiying Zhang

Frontiers in Genetics

doi 10.3389/fgene.2019.00347

5,652 views
3 citations

Original Research

Published on 12 Apr 2019

Targeted, High-Resolution RNA Sequencing of Non-coding Genomic Regions Associated With Neuropsychiatric Functions

in Genomic Assay Technology

Simon A. Hardwick
Maya Bassett
Dominik Kaczorowski
James Blackburn
Kirston Barton
Nenad Bartonicek
Shaun L. Carswell
Hagen U. Tilgner
Clement Loy
Glenda Halliday

Frontiers in Genetics

doi 10.3389/fgene.2019.00309

14,671 views
34 citations

Review

Published on 21 Mar 2019

Analysis of Transcriptome and Epitranscriptome in Plants Using PacBio Iso-Seq and Nanopore-Based Direct RNA Sequencing

in Genomic Assay Technology

Liangzhen Zhao
Hangxiao Zhang
Markus V. Kohnen
Kasavajhala V. S. K. Prasad
Lianfeng Gu
Anireddy S. N. Reddy

Frontiers in Genetics

doi 10.3389/fgene.2019.00253

43,960 views
144 citations

Original Research

Published on 15 Mar 2019

Programmable Base Editing of the Sheep Genome Revealed No Genome-Wide Off-Target Mutations

in Genomic Assay Technology

Shiwei Zhou
Bei Cai
Chong He
Ying Wang
Qiang Ding
Jiao Liu
Yao Liu
Yige Ding
Xiaoe Zhao
Guanwei Li

Frontiers in Genetics

doi 10.3389/fgene.2019.00215

8,666 views
37 citations

Protocols

Published on 13 Mar 2019

Guidelines for Fluorescent Guided Biallelic HDR Targeting Selection With PiggyBac System Removal for Gene Editing

in Genomic Assay Technology

Javier Jarazo
Xiaobing Qing
Jens C. Schwamborn

Frontiers in Genetics

doi 10.3389/fgene.2019.00190

7,909 views
9 citations

General Commentary

Published on 07 Mar 2019

Commentary: Leucine-rich Repeat Receptor-Like Gene Screen Reveals that Nicotiana RXEG1 Regulates Glycoside Hydrolase 12 MAMP Detection

in Genomic Assay Technology

Si Wu
Wei Wang
Xiangru Xu
Xiaolin Wu

Frontiers in Genetics

doi 10.3389/fgene.2019.00162

2,726 views
2 citations

Perspective

Published on 01 Mar 2019

The Impact of Heterogeneity on Single-Cell Sequencing

in Genomic Assay Technology

Samantha L. Goldman
Matthew MacKay
Ebrahim Afshinnekoo
Ari M. Melnick
Shuxiu Wu
Christopher E. Mason

Frontiers in Genetics

doi 10.3389/fgene.2019.00008

24,462 views
107 citations

Original Research

Published on 19 Feb 2019

Precise and Rapid Validation of Candidate Gene by Allele Specific Knockout With CRISPR/Cas9 in Wild Mice

in Genomic Assay Technology

Tianzhu Chao
Zhuangzhuang Liu
Yu Zhang
Lichen Zhang
Rong Huang
Le He
Yanrong Gu
Zhijun Chen
Qianqian Zheng
Lijin Shi

Frontiers in Genetics

doi 10.3389/fgene.2019.00124

6,369 views
15 citations

Original Research

Published on 21 Jan 2019

FTO Knockout Causes Chromosome Instability and G2/M Arrest in Mouse GC-1 Cells

in Genomic Assay Technology

Tao Huang
Qiang Gao
Tongying Feng
Yi Zheng
Jiayin Guo
Wenxian Zeng

Frontiers in Genetics

doi 10.3389/fgene.2018.00732

7,760 views
36 citations

Review

Published on 07 Jan 2019

Methodologies for Improving HDR Efficiency

in Genomic Assay Technology

Mingjie Liu
Saad Rehman
Xidian Tang
Kui Gu
Qinlei Fan
Dekun Chen
Wentao Ma

Frontiers in Genetics

doi 10.3389/fgene.2018.00691

101,397 views
283 citations

Original Research

Published on 04 Oct 2018

Trio-Based Deep Sequencing Reveals a Low Incidence of Off-Target Mutations in the Offspring of Genetically Edited Goats

in Genomic Assay Technology

Chao Li
Shiwei Zhou
Yan Li
Guanwei Li
Yige Ding
Lan Li
Jing Liu
Lei Qu
Tad Sonstegard
Xingxu Huang

Frontiers in Genetics

doi 10.3389/fgene.2018.00449

8,337 views
30 citations

Review

Published on 04 Sep 2018

Genome Editing of Pigs for Agriculture and Biomedicine

in Genomic Assay Technology

Huaqiang Yang
Zhenfang Wu

Frontiers in Genetics

doi 10.3389/fgene.2018.00360

49,720 views
81 citations