Frontiers in Genome Editing

Original Research

Published on 29 Aug 2025

CRISPR-FMC: a dual-branch hybrid network for predicting CRISPR-Cas9 on-target activity

in Genome Editing Tools and Mechanisms

Chuxuan Li
Jian Li
Quan Zou
Hailin Feng

Frontiers in Genome Editing

doi 10.3389/fgeed.2025.1643888

1,892 views

Brief Research Report

Published on 28 Aug 2025

Prime editing using paired pegRNAs targeting NG- or NGG-PAM in rice

in Genome Editing in Plants

Ayako Nishizawa-Yokoi
Keiko Iida
Akiko Mori
Seiichi Toki

Frontiers in Genome Editing

doi 10.3389/fgeed.2025.1550308

2,138 views

Review

Published on 26 Aug 2025

Stem cell and CRISPR/Cas9 gene editing technology in Alzheimer’s disease therapy: from basic research to clinical innovation

in Genome Editing in Human Health and Disease

Cong He
Baojiang Chen
Ciai Yan
Xiaoqing Zhou

Frontiers in Genome Editing

doi 10.3389/fgeed.2025.1612868

6,956 views
2 citations

Perspective

Published on 20 Aug 2025

Ethical dimensions and societal implications: ensuring the social responsibility of CRISPR technology

in Genome Editing in Human Health and Disease

Irfan Biswas

Frontiers in Genome Editing

doi 10.3389/fgeed.2025.1593172

12,778 views
2 citations

Editorial

Published on 31 Jul 2025

Editorial: Gene editing to achieve Zero Hunger

in Genome Editing in Plants

Shakeel Ahmad
Iqrar Ahmad Rana
Kevin M. Folta
Christian Damian Lorenzo
Sultan Habibullah Khan

Frontiers in Genome Editing

doi 10.3389/fgeed.2025.1632120

4,011 views
1 citation

Original Research

Published on 24 Jul 2025

CATS: a bioinformatic tool for automated Cas9 nucleases activity comparison in clinically relevant contexts

in Genome Editing Tools and Mechanisms

Ettore Rocchi
Federico Magnani
Gastone Castellani
Antonio Carusillo
Martina Tarozzi

Frontiers in Genome Editing

doi 10.3389/fgeed.2025.1571023

2,324 views

Brief Research Report

Published on 23 Jul 2025

CRISPR-Cas9 editing of TNFAIP3 variants in salivary gland epithelial cells to study Sjögren’s disease pathogenesis

in Genome Editing in Human Health and Disease

Subhashis Ghosh
Qisheng Tu
Zoe Xiaofang Zhu
Sreelakshmi Panginikkod
Jake Jinkun Chen

Frontiers in Genome Editing

doi 10.3389/fgeed.2025.1625393

1,959 views

Original Research

Published on 02 Jul 2025

Simply cut out – Combining CRISPR/Cas9 RNPs and transiently selected telomere vectors for marker free-gene deletion in Trichoderma atroviride

in Genome Editing Tools and Mechanisms

Mario Gründlinger
Chiara Ellensohn
Leo Drechsel
Ulrike Schreiner
Siebe Pierson
Clara Baldin
Susanne Zeilinger

Frontiers in Genome Editing

doi 10.3389/fgeed.2025.1623963

2,490 views

Methods

Published on 01 Jul 2025

Comprehensive approaches to design efficient gRNA for SDN1-CRISPR/Cas9 genome editing in wheat

in Genome Editing in Plants

Tushadri Singh
H. M. Mamrutha
Rajender Singh
J. P. Jaiswal
Zeenat Wadhwa
Rakesh Kumar
Omvir Singh
O. P. Ahlawat
Ratan Tiwari

Frontiers in Genome Editing

doi 10.3389/fgeed.2025.1579165

3,460 views

Review

Published on 30 Jun 2025

Genome editing for sustainable agriculture in Peru: advances, potential applications and regulation

in Genome Editing in Plants

Marilu Mestanza
Angel David Hernández-Amasifuen
Alexandra Jherina Pineda-Lázaro
Dennis Eriksson
Juan Carlos Guerrero-Abad

Frontiers in Genome Editing

doi 10.3389/fgeed.2025.1611040

5,172 views
2 citations

Brief Research Report

Published on 30 Jun 2025

DNA-free CRISPR genome editing in raspberry (Rubus idaeus) protoplast through RNP-mediated transfection

in Genome Editing in Plants

Ryan Creeth
Andrew Thompson
Zoltan Kevei

Frontiers in Genome Editing

doi 10.3389/fgeed.2025.1589431

5,192 views

Brief Research Report

Published on 18 Jun 2025

A long shelf-life melon created via CRISPR/Cas9 RNP-based in planta genome editing

in Genome Editing in Plants

Kentaro Sasaki
Kaoru Urano
Naozumi Mimida
Satoko Nonaka
Hiroshi Ezura
Ryozo Imai

Frontiers in Genome Editing

doi 10.3389/fgeed.2025.1623097

3,670 views

Original Research

Published on 04 Jun 2025

Long read sequencing reveals transgene concatemerization and vector sequences integration following AAV-driven electroporation of CRISPR RNP complexes in mouse zygotes

in Genome Editing in Animals

Muhammad W. Luqman
Piroon Jenjaroenpun
Jessica Spathos
Nikhil Shingte
Mitchell Cummins
Pattaraporn Nimsamer
Lars M. Ittner
Thidathip Wongsurawat
Fabien Delerue

Frontiers in Genome Editing

doi 10.3389/fgeed.2025.1582097

4,491 views
5 citations

Review

Published on 03 Jun 2025

Recent developments, challenges and opportunities in genome editing for crop science from a societal perspective

in Genome Editing in Plants

Srividhya Venkataraman
David Zaruk
Kathleen Hefferon

Frontiers in Genome Editing

doi 10.3389/fgeed.2025.1568072

6,505 views
1 citation

Methods

Published on 30 May 2025

Lentiviral CRISPRa/i in the adult prairie vole brain: modulating neuronal gene expression without DNA cleavage

in Genome Editing in Animals

Meredith K. Loth
Kendall T. Mesch
Celine Herrera-Garcia
Liza E. Brusman
Zoe R. Donaldson

Frontiers in Genome Editing

doi 10.3389/fgeed.2025.1602983

2,853 views
1 citation

Editorial

Published on 22 May 2025

Editorial: Plant breeding innovations—CRISPR as a powerful weapon for agricultural crops

in Genome Editing in Plants

Basavantraya N. Devanna
Yugander Arra
Maganti Sheshu Madhav

Frontiers in Genome Editing

doi 10.3389/fgeed.2025.1623540

4,667 views

Review

Published on 21 May 2025

Base editors in zebrafish: a new era for functional genomics and disease modeling

in Genome Editing in Animals

Yuwen Liu
Chao Li
Yiren Qiu
Sihong Chen
Yijun Luo
Donghua Xiong
Jun Zhao
Jianmin Ye
Xuegeng Wang
Wei Qin

Frontiers in Genome Editing

doi 10.3389/fgeed.2025.1598887

4,466 views
1 citation

Review

Published on 20 May 2025

Genome editing in grain legumes for food security

in Genome Editing in Plants

Joshua Yeboah Asiamah
Sakina Haruna Mahdi
Kusum R. Tamang
Christian Bryan Carson
Prabesh Koirala
Emily Anne Reed
Aaron Tettey Asare
Anu Augustine
Milind B. Ratnaparkhe
Kailash C. Bansal

Frontiers in Genome Editing

doi 10.3389/fgeed.2025.1572292

6,123 views
2 citations

Original Research

Published on 30 Apr 2025

Efficient GBA1 editing via HDR with ssODNs by outcompeting pseudogene-mediated gene conversion upon CRISPR/Cas9 cleavage

in Genome Editing in Human Health and Disease

Joseph S. Lagas
Monica F. Sentmanat
Xiaoxia Cui

Frontiers in Genome Editing

doi 10.3389/fgeed.2025.1581743

3,875 views
1 citation

Review

Published on 11 Apr 2025

Unraveling the future of genomics: CRISPR, single-cell omics, and the applications in cancer and immunology

in Genome Editing in Cancer and Immunology

A. Vipin Menon
Bicna Song
Lumen Chao
Diksha Sriram
Pamela Chansky
Ishnoor Bakshi
Jane Ulianova
Wei Li

Frontiers in Genome Editing

doi 10.3389/fgeed.2025.1565387

12,425 views
6 citations

Original Research

Published on 04 Apr 2025

Mapping the therapeutic landscape of CRISPR-Cas9 for combating age-related diseases

in Genome Editing in Human Health and Disease

Qiyu He
Yida Wang
Zhimin Tan
Xian Zhang
Chao Yu
Xiaoqin Jiang

Frontiers in Genome Editing

doi 10.3389/fgeed.2025.1558432

5,013 views
2 citations

Mini Review

Published on 02 Apr 2025

CRISPR-dependent base editing as a therapeutic strategy for rare monogenic disorders

in Genome Editing Tools and Mechanisms

Júlia-Jié Cabré-Romans
Raquel Cuella-Martin

Frontiers in Genome Editing

doi 10.3389/fgeed.2025.1553590

9,300 views
4 citations

Brief Research Report

Published on 01 Apr 2025

Cas9 endonuclease: a molecular tool for in vitro cloning and CRISPR edit detection

in Genome Editing in Plants

Xingliang Ma
Dhouha Kthiri
Manpartik S. Gill
Curtis J. Pozniak
Sateesh Kagale

Frontiers in Genome Editing

doi 10.3389/fgeed.2025.1565297

3,238 views
1 citation

Review

Published on 18 Mar 2025

CRISPR/Cas9: a sustainable technology to enhance climate resilience in major Staple Crops

in Genome Editing in Plants

Navjot Kaur
Muslim Qadir
Dali V. Francis
Anshu Alok
Siddharth Tiwari
Zienab F. R. Ahmed

Frontiers in Genome Editing

doi 10.3389/fgeed.2025.1533197

13,051 views
13 citations

287 articles

articles

CRISPR-FMC: a dual-branch hybrid network for predicting CRISPR-Cas9 on-target activity

Prime editing using paired pegRNAs targeting NG- or NGG-PAM in rice

Stem cell and CRISPR/Cas9 gene editing technology in Alzheimer’s disease therapy: from basic research to clinical innovation

Ethical dimensions and societal implications: ensuring the social responsibility of CRISPR technology

Editorial: Gene editing to achieve Zero Hunger

CATS: a bioinformatic tool for automated Cas9 nucleases activity comparison in clinically relevant contexts

CRISPR-Cas9 editing of TNFAIP3 variants in salivary gland epithelial cells to study Sjögren’s disease pathogenesis

Simply cut out – Combining CRISPR/Cas9 RNPs and transiently selected telomere vectors for marker free-gene deletion in Trichoderma atroviride

Comprehensive approaches to design efficient gRNA for SDN1-CRISPR/Cas9 genome editing in wheat

Genome editing for sustainable agriculture in Peru: advances, potential applications and regulation

DNA-free CRISPR genome editing in raspberry (Rubus idaeus) protoplast through RNP-mediated transfection

A long shelf-life melon created via CRISPR/Cas9 RNP-based in planta genome editing

Long read sequencing reveals transgene concatemerization and vector sequences integration following AAV-driven electroporation of CRISPR RNP complexes in mouse zygotes

Recent developments, challenges and opportunities in genome editing for crop science from a societal perspective

Lentiviral CRISPRa/i in the adult prairie vole brain: modulating neuronal gene expression without DNA cleavage

Editorial: Plant breeding innovations—CRISPR as a powerful weapon for agricultural crops

Base editors in zebrafish: a new era for functional genomics and disease modeling

Genome editing in grain legumes for food security

Efficient GBA1 editing via HDR with ssODNs by outcompeting pseudogene-mediated gene conversion upon CRISPR/Cas9 cleavage

Unraveling the future of genomics: CRISPR, single-cell omics, and the applications in cancer and immunology

Mapping the therapeutic landscape of CRISPR-Cas9 for combating age-related diseases

CRISPR-dependent base editing as a therapeutic strategy for rare monogenic disorders

Cas9 endonuclease: a molecular tool for in vitro cloning and CRISPR edit detection

CRISPR/Cas9: a sustainable technology to enhance climate resilience in major Staple Crops