Frontiers in Genome Editing

Original Research

Published on 02 Jul 2025

Simply cut out – Combining CRISPR/Cas9 RNPs and transiently selected telomere vectors for marker free-gene deletion in Trichoderma atroviride

Mario Gründlinger
Chiara Ellensohn
Leo Drechsel
Ulrike Schreiner
Siebe Pierson
Clara Baldin
Susanne Zeilinger

Frontiers in Genome Editing

doi 10.3389/fgeed.2025.1623963

501 views

Methods

Published on 01 Jul 2025

Comprehensive approaches to design efficient gRNA for SDN1-CRISPR/Cas9 genome editing in wheat

Tushadri Singh
H. M. Mamrutha
Rajender Singh
J. P. Jaiswal
Zeenat Wadhwa
Rakesh Kumar
Omvir Singh
O. P. Ahlawat
Ratan Tiwari

Frontiers in Genome Editing

doi 10.3389/fgeed.2025.1579165

672 views

Brief Research Report

Published on 30 Jun 2025

DNA-free CRISPR genome editing in raspberry (Rubus idaeus) protoplast through RNP-mediated transfection

Ryan Creeth
Andrew Thompson
Zoltan Kevei

Frontiers in Genome Editing

doi 10.3389/fgeed.2025.1589431

715 views

Review

Published on 30 Jun 2025

Genome editing for sustainable agriculture in Peru: advances, potential applications and regulation

Marilu Mestanza
Angel David Hernández-Amasifuen
Alexandra Jherina Pineda-Lázaro
Dennis Eriksson
Juan Carlos Guerrero-Abad

Frontiers in Genome Editing

doi 10.3389/fgeed.2025.1611040

1,099 views

Brief Research Report

Accepted on 24 Jun 2025

Prime editing using paired pegRNAs targeting NG-or NGG-PAM in rice

Ayako Nishizawa-Yokoi
Keiko Iida
Akiko Mori
Seiichi Toki

Frontiers in Genome Editing

doi 10.3389/fgeed.2025.1550308

233 views

Brief Research Report

Accepted on 23 Jun 2025

"CRISPR-Cas9 Editing of TNFAIP3 Variants in Salivary Gland Epithelial Cells to Study Sjögren's Disease Pathogenesis"

Subhashis Ghosh
Qisheng Tu
Zoe Xiaofang Zhu
Sreelakshmi Panginikkod
Jake Chen

Frontiers in Genome Editing

doi 10.3389/fgeed.2025.1625393

318 views

Brief Research Report

Published on 18 Jun 2025

A long shelf-life melon created via CRISPR/Cas9 RNP-based in planta genome editing

Kentaro Sasaki
Kaoru Urano
Naozumi Mimida
Satoko Nonaka
Hiroshi Ezura
Ryozo Imai

Frontiers in Genome Editing

doi 10.3389/fgeed.2025.1623097

1,077 views

Original Research

Published on 04 Jun 2025

Long read sequencing reveals transgene concatemerization and vector sequences integration following AAV-driven electroporation of CRISPR RNP complexes in mouse zygotes

Muhammad W. Luqman
Piroon Jenjaroenpun
Jessica Spathos
Nikhil Shingte
Mitchell Cummins
Pattaraporn Nimsamer
Lars M. Ittner
Thidathip Wongsurawat
Fabien Delerue

Frontiers in Genome Editing

doi 10.3389/fgeed.2025.1582097

1,813 views

Review

Published on 03 Jun 2025

Recent developments, challenges and opportunities in genome editing for crop science from a societal perspective

Srividhya Venkataraman
David Zaruk
Kathleen Hefferon

Frontiers in Genome Editing

doi 10.3389/fgeed.2025.1568072

1,577 views

Methods

Published on 30 May 2025

Lentiviral CRISPRa/i in the adult prairie vole brain: modulating neuronal gene expression without DNA cleavage

Meredith K. Loth
Kendall T. Mesch
Celine Herrera-Garcia
Liza E. Brusman
Zoe R. Donaldson

Frontiers in Genome Editing

doi 10.3389/fgeed.2025.1602983

1,129 views

Editorial

Published on 22 May 2025

Editorial: Plant breeding innovations—CRISPR as a powerful weapon for agricultural crops

Basavantraya N. Devanna
Yugander Arra
Maganti Sheshu Madhav

Frontiers in Genome Editing

doi 10.3389/fgeed.2025.1623540

1,717 views

Review

Published on 21 May 2025

Base editors in zebrafish: a new era for functional genomics and disease modeling

Yuwen Liu
Chao Li
Yiren Qiu
Sihong Chen
Yijun Luo
Donghua Xiong
Jun Zhao
Jianmin Ye
Xuegeng Wang
Wei Qin

Frontiers in Genome Editing

doi 10.3389/fgeed.2025.1598887

1,616 views

Review

Published on 20 May 2025

Genome editing in grain legumes for food security

Joshua Yeboah Asiamah
Sakina Haruna Mahdi
Kusum R. Tamang
Christian Bryan Carson
Prabesh Koirala
Emily Anne Reed
Aaron Tettey Asare
Anu Augustine
Milind B. Ratnaparkhe
Kailash C. Bansal

Frontiers in Genome Editing

doi 10.3389/fgeed.2025.1572292

2,536 views

Original Research

Published on 30 Apr 2025

Efficient GBA1 editing via HDR with ssODNs by outcompeting pseudogene-mediated gene conversion upon CRISPR/Cas9 cleavage

Joseph S. Lagas
Monica F. Sentmanat
Xiaoxia Cui

Frontiers in Genome Editing

doi 10.3389/fgeed.2025.1581743

1,819 views

Perspective

Accepted on 24 Apr 2025

Ethical Dimensions and Societal Implications: Ensuring the Social Responsibility of CRISPR Technology

Irfan Biswas

Frontiers in Genome Editing

doi 10.3389/fgeed.2025.1593172

650 views

Review

Published on 11 Apr 2025

Unraveling the future of genomics: CRISPR, single-cell omics, and the applications in cancer and immunology

A. Vipin Menon
Bicna Song
Lumen Chao
Diksha Sriram
Pamela Chansky
Ishnoor Bakshi
Jane Ulianova
Wei Li

Frontiers in Genome Editing

doi 10.3389/fgeed.2025.1565387

3,622 views