Frontiers in Toxicology | In Vitro Toxicology

Original Research

Published on 18 Dec 2025

Beyond cytotoxicity: pollutant mixtures elicit unconventional epithelial-fibroblast signaling in a human lung air-liquid interface co-culture model

in In Vitro Toxicology

Justine Fredoc-Louison
Maëva Cherrière
Bastien Rival
Suzanne De Araujo
Sabine François
Samir Dekali

Frontiers in Toxicology

doi 10.3389/ftox.2025.1722968

441 views

Mini Review

Accepted on 15 Dec 2025

Beyond the amyloid hypothesis: Leveraging human-centered complex in vitro models to decode Alzheimer's disease etiology

in In Vitro Toxicology

Matthew Price
Francesca Pistollato

Frontiers in Toxicology

314 views

Original Research

Published on 08 Dec 2025

Comparative evaluation of artificial saliva and complete artificial saliva as solvent vehicles for in vitro toxicity testing of oral tobacco products

in In Vitro Toxicology

Xuefei Cao
Mariana T. Farcas
Yevgeniya V. Prepelitskaya
Abaigeal Ritzenthaler
Jennifer Molignano
Jonathan Oldach
Marisol M. Gutierrez

Frontiers in Toxicology

doi 10.3389/ftox.2025.1657073

710 views

Original Research

Published on 04 Dec 2025

Monocyte migration assay using a vascular-on-a-chip model and its utilization for the evaluation of a heated tobacco product

in In Vitro Toxicology

Ayaka Hayashida
Atsuko Nozawa
Shigeaki Ito

Frontiers in Toxicology

doi 10.3389/ftox.2025.1658093

756 views

Review

Published on 02 Dec 2025

Advancements in the in vitro culture of human pluripotent stem cells: progress, challenges, and future directions: comprehensive review

in In Vitro Toxicology

Niraj Chaudhary
Luis G. Villa-Diaz

Frontiers in Toxicology

doi 10.3389/ftox.2025.1667573

1,143 views

Original Research

Accepted on 01 Dec 2025

Nicotine-free electronic vape fluid stimulates angiogenic processes in vitro through ARF6-mediated oxidative stress

in In Vitro Toxicology

Lewis Spurrier-Best
David Butcher
Evangelene Blackham-Hayward
Zsuzsanna Kertesz
Havovi Chichger

Frontiers in Toxicology

278 views

Opinion

Published on 14 Nov 2025

Revisiting 3Rs: rethinking replacement and new approach methodologies

in In Vitro Toxicology

Jessica Rosolowski
Tilo Weber
Atena Malakpour-Permlid
Stina Oredsson

Frontiers in Toxicology

doi 10.3389/ftox.2025.1664209

1,212 views

Review

Published on 30 Oct 2025

Developing an approach for evaluating the cardiotoxic potential of botanicals

in In Vitro Toxicology

Julie Krzykwa
Hemantkumar S. Chaudhari
Andre Monteiro Da Rocha
Matthias Gossmann
Peter Hoffmann
Yaser Khokhar
Nathan Meyer
Jin-Young K. Park
Robert Sprando
Ravi Vaidyanathan

Frontiers in Toxicology

doi 10.3389/ftox.2025.1646044

1,888 views
1 citation

Original Research

Published on 30 Oct 2025

Alternatives to animal-derived extracellular matrix hydrogels? An explorative study with HepaRG cells in animal-free hydrogels under static and dynamic culture conditions

in In Vitro Toxicology

Katharina S. Nitsche
Paul L. Carmichael
Sophie Malcomber
Iris Müller
Hans Bouwmeester

Frontiers in Toxicology

doi 10.3389/ftox.2025.1649393

2,011 views
1 citation

Perspective

Published on 13 Oct 2025

Refining the safety assessment of newly expressed proteins in GMOs

in In Vitro Toxicology

Elena Sánchez-Brunete
Arianna Ferrari
F. Javier Moreno
Tilemachos Goumperis
Michele Ardizzone
Reinhilde Schoonjans
Ian Dewhurst
Ivan Dimitrov
Michelle M. Epstein
Frits Koning

Frontiers in Toxicology

doi 10.3389/ftox.2025.1679506

1,875 views

Original Research

Published on 03 Oct 2025

Characterizing chlorotriazine effects in cancer-relevant high-throughput screening assays

in In Vitro Toxicology

Agnes L. Karmaus
Alex Charlton

Frontiers in Toxicology

doi 10.3389/ftox.2025.1682439

1,103 views

Original Research

Published on 01 Oct 2025

Proliferation and metabolic activity of the Atlantic sturgeon cell line AOXlar7y under short-term serum-reduced conditions, and the effect of stimulation with growth factors and cytokines

in In Vitro Toxicology

Valeria Di Leonardo
Julia Brenmoehl
Heike Wanka
Bianka Grunow

Frontiers in Toxicology

doi 10.3389/ftox.2025.1636776

1,495 views

Original Research

Published on 01 Oct 2025

Promoting ethical and reproducible cell culture: implementing animal-free alternatives to teaching in molecular and cell biology

in In Vitro Toxicology

Alexandra Nessar
Viola Röhrs
Mathias Ziersch
Ahmed S. M. Ali
Julia Moradi
Anke Kurreck
Johanna Berg
Jens Kurreck

Frontiers in Toxicology

doi 10.3389/ftox.2025.1670513

4,276 views
1 citation

Original Research

Published on 30 Sep 2025

Acetaldehyde and methylglyoxal: comparative analysis of toxic electronic cigarette degradation products in 3D and 2D exposure systems using human bronchial epithelial models

in In Vitro Toxicology

Man Wong
Teresa Martinez
Nathan Hendricks
Prue Talbot

Frontiers in Toxicology

doi 10.3389/ftox.2025.1624794

4,013 views

Review

Published on 09 Sep 2025

Antibody-based alternatives to animal testing for toxin detection and antitoxin evaluation

in In Vitro Toxicology

Seo-Hyuk Chang
Jieun Jang
Wonjun Yang
Nam-Kyung Lee
Seoyeon Choi
Hyo-Il Jung
Wantae Kim
Boksik Cha
Sung-Jin Yoon
Ji-Yoon Noh

Frontiers in Toxicology

doi 10.3389/ftox.2025.1636246

2,727 views

Original Research

Published on 21 Aug 2025

Integral approach to organelle profiling in human iPSC-derived cardiomyocytes enhances in vitro cardiac safety classification of known cardiotoxic compounds

in In Vitro Toxicology

Brigitta R. Szabo
Jeroen Stein
Anna Savchenko
Thomas Hutschalik
Filip Van Nieuwerburgh
Tim Meese
Georgios Kosmidis
Paul G. A. Volders
Elena Matsa

Frontiers in Toxicology

doi 10.3389/ftox.2025.1644119

2,138 views

Review

Published on 08 Aug 2025

Fetal bovine serum: how to leave it behind in the pursuit of more reliable science

in In Vitro Toxicology

Tilo Weber
Atena Malakpour-Permlid
Aline Chary
Vito D’Alessandro
Leah Haut
Sebastian Seufert
Esther Veronika Wenzel
James Hickman
Karen Bieback
Joachim Wiest

Frontiers in Toxicology

doi 10.3389/ftox.2025.1612903

9,390 views
6 citations

Original Research

Published on 23 Jul 2025

Cadmium decreases human gingival fibroblast viability and induces pro-inflammatory response associated with Akt and MAPK pathway activation

in In Vitro Toxicology

Tipparat Parakaw
Sirada Srihirun
Nathawut Sibmooh
Nisarat Ruangsawasdi
Phisit Khemawoot
Pornpun Vivithanaporn

Frontiers in Toxicology

doi 10.3389/ftox.2025.1583865

2,285 views
1 citation

Original Research

Published on 01 Jul 2025

Toxicity of glyphosate accelerates neurodegeneration in Caenorhabditis elegans model of Alzheimer’s disease

in In Vitro Toxicology

Nisha Rani
Mohammad Mumtaz Alam
Suhel Parvez

Frontiers in Toxicology

doi 10.3389/ftox.2025.1578230

2,490 views
2 citations

Review

Published on 30 Jun 2025

An adverse outcome pathway for cigarette smoke-mediated oxidative stress in plaque formation

in In Vitro Toxicology

Patrudu Makena
Linsey E. Haswell
Michael McEwan
Brian M. Keyser
David E. Smart
Robert Leverette
Kristen Jordan
Damien Breheny
Sarah Baxter-Wright

Frontiers in Toxicology

doi 10.3389/ftox.2025.1554747

2,481 views
2 citations

Systematic Review

Published on 27 Jun 2025

Towards the standardization of human platelet lysate production and its comparison to fetal bovine serum for human hematopoietic cell culture: a scoping review

in In Vitro Toxicology

Shamili Immalaraju
Srishti Goyal
Rukmini Jonnalagadda

Frontiers in Toxicology

doi 10.3389/ftox.2025.1496231

3,370 views
2 citations

Original Research

Published on 23 Jun 2025

An injectable 2.5% cross-linked polyacrylamide hydrogel (2.5 iPAAG) demonstrates no neurotoxicity in human induced pluripotent stem cells-derived iCell® GlutaNeurons

in In Vitro Toxicology

Peter S. Walmod
Philip Kusk
Nina Jøhnk
Ieva Ankorina-Stark
Anthony Essex

Frontiers in Toxicology

doi 10.3389/ftox.2025.1585430

2,589 views

Original Research

Published on 12 Jun 2025

Bioengineering of novel organotypic 3D human liver tissue model for drug-induced liver injury and toxicity studies

in In Vitro Toxicology

Camden Holm
Joseph Finelli
Mateo Frare
Alex Armento
Seyoum Ayehunie

Frontiers in Toxicology

doi 10.3389/ftox.2025.1574387

3,086 views

Original Research

Published on 15 May 2025

Quantitative adverse outcome pathway modeling for cigarette-smoke-induced airway mucus hypersecretion. Part 1: adverse-outcome-pathway-based in vitro assessment with repeated exposure to whole cigarette smoke

in In Vitro Toxicology

Sakuya Ichikawa
Shugo Muratani
Keigo Sano
Kazuo Erami
Akina Mori
Risa Matsumoto
Shigeaki Ito

Frontiers in Toxicology

doi 10.3389/ftox.2025.1564857

1,952 views
1 citation

100 articles

articles

Beyond cytotoxicity: pollutant mixtures elicit unconventional epithelial-fibroblast signaling in a human lung air-liquid interface co-culture model

Beyond the amyloid hypothesis: Leveraging human-centered complex in vitro models to decode Alzheimer's disease etiology

Comparative evaluation of artificial saliva and complete artificial saliva as solvent vehicles for in vitro toxicity testing of oral tobacco products

Monocyte migration assay using a vascular-on-a-chip model and its utilization for the evaluation of a heated tobacco product

Advancements in the in vitro culture of human pluripotent stem cells: progress, challenges, and future directions: comprehensive review

Nicotine-free electronic vape fluid stimulates angiogenic processes in vitro through ARF6-mediated oxidative stress

Revisiting 3Rs: rethinking replacement and new approach methodologies

Developing an approach for evaluating the cardiotoxic potential of botanicals

Alternatives to animal-derived extracellular matrix hydrogels? An explorative study with HepaRG cells in animal-free hydrogels under static and dynamic culture conditions

Refining the safety assessment of newly expressed proteins in GMOs

Characterizing chlorotriazine effects in cancer-relevant high-throughput screening assays

Proliferation and metabolic activity of the Atlantic sturgeon cell line AOXlar7y under short-term serum-reduced conditions, and the effect of stimulation with growth factors and cytokines

Promoting ethical and reproducible cell culture: implementing animal-free alternatives to teaching in molecular and cell biology

Acetaldehyde and methylglyoxal: comparative analysis of toxic electronic cigarette degradation products in 3D and 2D exposure systems using human bronchial epithelial models

Antibody-based alternatives to animal testing for toxin detection and antitoxin evaluation

Integral approach to organelle profiling in human iPSC-derived cardiomyocytes enhances in vitro cardiac safety classification of known cardiotoxic compounds

Fetal bovine serum: how to leave it behind in the pursuit of more reliable science

Cadmium decreases human gingival fibroblast viability and induces pro-inflammatory response associated with Akt and MAPK pathway activation

Toxicity of glyphosate accelerates neurodegeneration in Caenorhabditis elegans model of Alzheimer’s disease

An adverse outcome pathway for cigarette smoke-mediated oxidative stress in plaque formation

Towards the standardization of human platelet lysate production and its comparison to fetal bovine serum for human hematopoietic cell culture: a scoping review

An injectable 2.5% cross-linked polyacrylamide hydrogel (2.5 iPAAG) demonstrates no neurotoxicity in human induced pluripotent stem cells-derived iCell® GlutaNeurons

Bioengineering of novel organotypic 3D human liver tissue model for drug-induced liver injury and toxicity studies

Quantitative adverse outcome pathway modeling for cigarette-smoke-induced airway mucus hypersecretion. Part 1: adverse-outcome-pathway-based in vitro assessment with repeated exposure to whole cigarette smoke